CD8 antibody (PE)

Catalog No. ABIN120398

The Mouse Monoclonal anti-CD8 antibody has been validated for FACS. It is suitable to detect CD8 in samples from Rat.

Quick Overview for CD8 antibody (PE) (ABIN120398)

Target: CD8

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: OX-8

Product Details anti-CD8 Antibody (PE)

Specificity: This antibody recognises the CD8 alpha cell surface antigen, expressed by a subset of T lymphocytes, most thymocytes and the majority of NK cells. Clone MRC OX-8 is suitable for use in in vitro blocking studies. We recommend the use of SM259A for this purpose. This clone has been described reacting with paraffin-embedded material following PLP Fixation (periodatelysine paraformaldehyde)

Purification: Affinity chromatography on Protein G

Immunogen: Rat thymocyte membrane glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Neat - 1/10, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL distilled water

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: RT/4 °C/-20 °C

Storage Comment: Lyophilized: Store at 2-8 °C (preferably in a dessicator). Reconstituted: Aliquot and store at -20 °C.

Target Details for CD8

Target: CD8

Alternative Name: CD8

Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

Gene ID: 24930

NCBI Accession: NP_113726

UniProt: P07725